… mechanism of protein export and provide fundamental unique insight into how bacteria target
… Cell and sec samples were analyzed for proteins of interest by SDS-PAGE and western …

… secretion system directly secretes proteins from bacterial cytosol to … system are first exported
to the periplasm through the Sec or Tat system, and then crossing outer membranes through …

… The Sec and Tat machineries in the cytoplasmic membrane serve as general … proteins
in Gram-positive bacteria, and to export proteins to the periplasm in Gram-negative bacteria (…

… spanning Sec and twin arginine translocation (Tat) systems which are … protein export, if it
had the banding pattern of the supernatant would look more like that of the lysates. The second …

… classic Sec system in which translocation occurs through an … include membrane protein
insertion by YidC in bacteria and … into lipid, and only the other part of the hairpin is translocated …

… from one species of bacteria to another. However, the … protein that is functionally translocated
by both export pathways … to engage with the Sec system for translocation into the periplasm, …

… within proteins and thus break down their substrates into … , bacteria have developed several
mechanisms for protein … across all kingdoms of life are the “general secretion” (Sec) and “…

… In addition, many proteins exported by the UT mechanism … bacteria, the folding of exported
proteins can also be stabilized by other types of covalent linkages between amino acid side …

… Using a model spidroin MaSpI16 composed of 16 consensus … the general Sec protein
export pathway for its secretion via N-… The bacterial strains and plasmids used in this study are …

… export gate subunits to allow cargo to enter the export apparatus. We also demonstrate through
… Many pathogenic Gram-negative bacteria utilize T3SS to export virulence factors in a well…